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Department of Internal Medicine, Section of Infectious Diseases, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.
We have developed a simplified protocol based on nested polymerase chain reaction (PCR) for early diagnosis of tuberculous meningitis Using this protocol, we detected the Mycobacterium tuberculosis genome within 24 hours in the CSF of 19 of 21 patients (90%) with clinically suspected tuberculous meningitis. The PCR results were negative in all 79 nontuberculous meningitis controls.
Address correspondence and reprint requests to Dr. Peter Yuk-Fong Liu, Department of Internal Medicine, Taichung Veterans General Hospital, 160 Taichung Harbour Road, Section 3, Taichung, Taiwan, R.O.C.
Received November 10, 1993. Accepted for publication in final form December 23, 1993.
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