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Immunoblot detection of oligoclonal anti-myelin basic protein IgG antibodies in cerebrospinal fluid in multiple sclerosis

Department of Neurology (Drs. CNZ, Olsson, Höjeberg, and Link), Karolinska Institute Medical School, Huddinge University Hospital, Stockholm, and the Department of Neurology (Dr. Emerudh), University of Linköping, Linköping, Sweden.

Migration properties and occurrence of antibodies against myelin basic protein (MBP) in paired CSF and serum specimens from patients with multiple sclerosis (MS) were demonstrated after agarose isoelectric focusing, immunoblot transfer, and immunoperoxidase staining. Oligoclonal IgG antibody bands directed against MBP were found in the CSF of 9 of 28 patients with MS (32%), but not in the CSF of any of 34 patients with other neurologic diseases. No serum showed anti-MBP antibody bands. The CSF anti-MBP antibodies migrated to the anodal region of the IgG area in a different fashion from oligoclonal IgG and anti-measles IgG antibodies, which were detected in parallel. The anti-MBP bands were transient in three of seven patients whom we studied consecutively. Enzyme-linked immunosorbent assay (ELISA) of serum and CSF for detection of IgG reactivity against MBP showed absorbance values above 2 standard deviations of controls in 44% of the MS patients and in 21% of those with other neurologic diseases. Results of this assay correlated partly with those of the immunoblot assay. ELISA positive and immunoblot negative results might be due to a broad polyclonal anti-MBP antibody response.

Address correspondence and reprint requests to Dr. Cruz, Department of Neurology, Huddinge University Hospital, S-141 86 Huddinge, Sweden.

Supported by grants from the Swedish Medical Research Council (Project nos. 3381 and 7488) and the Swedish Multiple Sclerosis Society.

Received October 24, 1986. Accepted for publication in final form April 21, 1986.

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