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NEUROLOGY 1987;37:309
© 1987 American Academy of Neurology

In situ analysis of neostriatal RNA and chromatin in Alzheimer's disease

J. A. Doebler, PhD, W. R. Markesbery, MD, A. Anthony, PhD and R. E. Rhoads, PhD

Departments of Biochemistry (Drs. Doebler and Rhoads) and Neurology and Pathology (Dr. Markesbery), University of Kentucky College of Medicine and Sanders-Brown Research Center on Aging, Lexington, KY; and the Department of Biology (Dr. Anthony), The Pennsylvania State University, University Park, PA.

Scanning-integrating microdensitometry of azure B-RNA-and Feulgen-Schiff-stained tissue sections was used to measure neostriatal neuronal RNA levels and susceptibility of neuronal and oligodendrocyte chromatin to acid hydrolysis in Alzheimer's disease (AD) patients and controls. AD was associated with neuronal RNA depletion (17 to 23%) in both caudate nucleus and putamen. While neuronal chromatin was found to be more acid-labile than that of the oligodendrocytes, there were no differences in either cell type between AD and controls. These data support the existence of a macromolecular disturbance (RNA loss) occurring within neostriatal neurons, perhaps related to the extrapyramidal dysfunction of AD, but fail to demonstrate that an alteration in chromatin is responsible for this effect.

Address correspondence and reprint requests to Dr. Rhoads, Department of Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536.

Received December 10, 1985. Accepted for publication June 6, 1986.

Supported by a Small Research Project Award to J.A. Doebler from the University of Kentucky Medical Center and by Grants IP01-AG05119 and IP50 AG05144 from the National Institutes of Health.






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