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The use of the chemical shift of the phosphomonoester P-31 magnetic resonance peak for the determination of intracellular pH in the brains of neonates

Department of Radiology (Drs. Corbett and Nunnally), Biological Magnetic Resonance Center, and the Department of Pediatrics (Dr. Laptook), University of Texas Health Science Center at Dallas, Dallas, TX.

The use of the chemical shift of the phosphomonoester P-31 magnetic resonance peak for the determination of intracellular pH has been assessed for piglet and neonatal human brain in vivo. The chemical shift difference between resonance peaks corresponding to phosphoethanolamine and inorganic phosphate, compared with phosphocreatine, was determined for piglets and human neonates. Using in vitro pH titration data to calculate intracellular pH, it was found that pH values from the phosphoethanolamine peak (pH 6.84 to 6.80) were lower than pH estimates from the inorganic phosphate peak (pH 7.22 to 6.99). This difference suggests that phosphoethanolamine and inorganic phosphate may exist in different intracellular environments. Results are presented to demonstrate that the phosphomonoester peak may also be used to measure changes in intracellular pH associated with brain ischemia.

Address correspondence and reprint requests to Dr. Corbett, Biological Magnetic Resonance Center, Department of Radiology, University of Texas Health Science Center at Dallas, Dallas, TX 75235.

Supported by funding from Diasonics (NMR) Inc. (R.L.N.), the National Institutes of Health Southwest In Vwo Resource Facility grant (R.L.N.), and National Institutes of Health grant R23–NS22398 (A.R.L.). Fellowship support (R.J.T.C.) was provided by the Department of Pediatrics and the Medical Research Council of Canada.

Received July 25, 1986. Accepted for publication in final form January 16, 1987.

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