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Anti-MAG antibody and antibody complexes

Detection by radioimmunoassay

Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY.

A radioimmunoassay (RIA) for measuring isotype-specific antibodies to the myelin-associated glycoprotein (MAG) was developed using radiolabeled CNS MAG in a double-antibody precipitation system. Anti-MAG activity was detected by RIA only in patients with neuropathy and anti-MAG M proteins. Anti-MAG IgM or IgG antibodies were not detected in serum of patients with Guillain-Barri. syndrome, chronic relapsing polyneuritis, or multiple sclerosis (MS). Some patients with anti-MAG IgM M proteins also had complexes of IgG or IgA bound to the M protein. In one patient, anti-CNS MAG activity was detected by RIA, but not by ELISA or immunoblot. Anti-MAG antibody activity in patients with neuropathy seems to be isotypically restricted, and there is no evidence for antibody reactivity to MAG in other demyelinating diseases.

Address correspondence and reprint requests to Dr. Latov, Department of Neurology, Columbia University College of Physicians and Surgeons, 630 West 168th Street. New York, NY 10032.

Supported by grants NS00659 and NS18016 from the National Institutes of Health and by grants from the Multiple Sclerosis Society and the Muscular Dystrophy Association. Dr. Nobile-Orazio is a visiting fellow from the Institute of Clinical Neurology, [Jniversity of Milan, Italy, and is the recipient of a fellowship from the National Multiple Sclerosis Society.

Accepted for publication October 26, 1984.

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