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Effects of amyotrophic lateral sclerosis sera on cultured cholinergic neurons

Department of Pharmacology, Centre Medical Universtaire, Geneva, Switzerland.

Dissociated monolayer cultures of chick ciliary ganglion neurons have been used to study the effects of control and ALS sera. The cultured neurons survive and extend neurites for a minimum of 2 weeks in a standard tissue culture medium that contains 10% heat-inactivated human serum. Three parameters of the neurons have been examined when cultured in control and ALS sera for 8 to 12 days: (1) neuronal survival, (2) activity of the enzyme choline acetyltransferase, and (3) synthesis of ³H-acetylcholine using ³H-choline as precursor. ALS sera cause a small decrease in these three parameters, but this difference is not significant.

Address correspondence and reprint requests to Dr. Kato. Department of Pharmacology, Centre Medical Universitaire, 1211 Geneve 4, Switzerland.

This work was supported by the Swiss National Fund for Scientific Research (No. 3.675.0.80) and the Foundation Schmidheiny. G.T. was supported for one year by the Swiss National Fund (No 3.489.0.791 to R.W. Straub, Department of Pharmacology, University of Geneva.

Accepted for publication, July 1, 1982.

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